P-91: Phenyl Hydrazine-Induced Anemia-Related Hypoxia Effect on Mice Testes Parameters:Implication for Protective Effect of Crocin

Background: There are reports indicating that among the others, hypoxia is one of the testes threatening factors, which may occur intentionally or accidentally. Hypoxia could be created secondarily after some pathological conditions such as anemia. In this study we aimed to investigate the crocin protective effect on the testes parameters in the animals that were exposed against phenyl hydrazine to induce anemia-dependent hypoxia. Materials and Methods: Forty nine male and clinically healthy mice (20-25 g and 6-8 weeks old) were divided to 7 groups including control and test groups. The anemia-dependent hypoxia was induced by using Phenyl hydrazine. The control (C) animals were received only saline normal during the experiment and the test groups were treated as follow: Phenyl hydrazine (PHN, 2 mg/100 g, 4mg/100g and 6 mg/100g) via IP.Three other groups revived crocin 40 mg/kg/day via IP. PHN was administered each 48 hours for 35 days. At the end of experiment testes were fixed in 10% formal saline and after tissue processing and staining with H&E method, samples were studied histomrphometricaly including capsule thickness, tubular diameter, thickness of the germinal epithelium, Sertoli and leydig cells number, tubular differentiation index (TDI), republication index(RI) and spermiogenesis index (SI) were investigated. Results: The results of this study showed that anemiarelated hypoxia resulted in a drastic reduction in germinal epithelium, Sertoli and leydig cells number, TDI, RI and SI, and Capsule thickness and tubular diameter did not change. While those groups which were treated with CRN showed a remarkable improvement in testes parameters. Conclusion: These results indicate a protective effect of CRN against the anemia-related hypoxia on testes. The protective capacity of CRN might relate to its known antioxidant properties.